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Organisme vivant modifié
(LMO)
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Enhanced grain yield potential and insect‑resistant maize, tolerant to herbicides glyphosate, glufosinate, 2,4‑Dichlorophenoxyacetic acid, and aryloxyphenoxypropionate
EN
DAS-Ø1131-3 x DP-91Ø521-2 x DP-2Ø2216-6 x DAS-4Ø278-9
Oui
DAS-Ø1131-3 x DP-91Ø521-2 x DP-2Ø2216-6 x DAS-4Ø278-9
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Organisation :CORTEVA AGRISCIENCE ARGENTINA S.R.L. ()Secteur privé (affaires et industrie)Avenida Libertador 101, Piso 1, 1638 Vicente López, Buenos Aires, ArgentinaBuenos Aires, Buenos Aires
, ArgentineTéléphone : Tel: 11-4110-0202 / 11-4110-0201,Télécopieur :E-mail : infoargentina@corteva.com,Site Web : https://www.corteva.com.ar/, -
Organisation :Corteva Agriscience ()Secteur privé (affaires et industrie)9330 Zionsville RoadIndianapolis, Indiana
46268, États-Unis d'AmériqueTéléphone : +1 833-267-8382,Télécopieur :E-mail : media@corteva.com,Site Web : https://www.corteva.com/,
The maize (Zea mays) was produced through cross‑breeding of modified parental varieties DAS‑Ø1131‑3, DP‑91Ø521‑2, DP‑2Ø2216‑6 and DAS‑4Ø278‑9.
Maize DAS‑Ø1131‑3 was genetically modified for resistance to Lepidopteran insect pests and tolerance to glyphosate herbicide. Resistance to insects is conferred by expression of a Bacillus thuringiensis chimeric delta endotoxin Cry1Da2, which forms non‑specific ion‑conducting pores in the apical membrane of midgut epithelial cells, leading to larval death. Tolerance to glyphosate is conferred by expression of a Streptomyces sviceus DGT28 5‑enolpyruvylshikimate‑3‑phosphate synthase (DGT28‑EPSPS), which allows the continued functioning of the shikimate pathway by avoiding inhibition from the herbicide.
Maize DP‑91Ø521‑2 was genetically modified for resistance to Lepidopteran insect pests and tolerance to glufosinate herbicide. Resistance to insects is conferred by expression of Bacillus thuringiensis Cry1B.34, which forms non‑specific ion‑conducting pores in the apical membrane of midgut epithelial cells, disrupting the midgut epithelium and leading to death. Tolerance to glufosinate is conferred by expression of phosphinothricin N‑acetyltransferase (PAT) from Streptomyces viridochromogenes, which detoxifies the herbicide by acetylation. In addition, this maize contains a phosphomannose isomerase (PMI) gene from Escherichia coli, used as a selectable marker during transformation.
Maize DP‑2Ø2216‑6 was genetically modified to confer enhanced grain yield potential and tolerance to glufosinate herbicide. Enhanced yield is associated with the constitutive expression of the Zea mays zmm28 gene, encoding a MADS‑box transcription factor involved in the regulation of genes associated with photosynthesis, nitrogen assimilation and carbon metabolism. Tolerance to glufosinate is conferred by expression of the pat gene from Streptomyces viridochromogenes.
Maize DAS‑4Ø278‑9 was genetically modified for tolerance to 2,4‑dichlorophenoxyacetic acid (2,4‑D) and aryloxyphenoxypropionate herbicides through expression of the Sphingobium herbicidovorans aryloxyalkanoate dioxygenase‑1 (aad‑1) gene, which inactivates these herbicides by oxidative cleavage.
Maize DAS‑Ø1131‑3 was genetically modified for resistance to Lepidopteran insect pests and tolerance to glyphosate herbicide. Resistance to insects is conferred by expression of a Bacillus thuringiensis chimeric delta endotoxin Cry1Da2, which forms non‑specific ion‑conducting pores in the apical membrane of midgut epithelial cells, leading to larval death. Tolerance to glyphosate is conferred by expression of a Streptomyces sviceus DGT28 5‑enolpyruvylshikimate‑3‑phosphate synthase (DGT28‑EPSPS), which allows the continued functioning of the shikimate pathway by avoiding inhibition from the herbicide.
Maize DP‑91Ø521‑2 was genetically modified for resistance to Lepidopteran insect pests and tolerance to glufosinate herbicide. Resistance to insects is conferred by expression of Bacillus thuringiensis Cry1B.34, which forms non‑specific ion‑conducting pores in the apical membrane of midgut epithelial cells, disrupting the midgut epithelium and leading to death. Tolerance to glufosinate is conferred by expression of phosphinothricin N‑acetyltransferase (PAT) from Streptomyces viridochromogenes, which detoxifies the herbicide by acetylation. In addition, this maize contains a phosphomannose isomerase (PMI) gene from Escherichia coli, used as a selectable marker during transformation.
Maize DP‑2Ø2216‑6 was genetically modified to confer enhanced grain yield potential and tolerance to glufosinate herbicide. Enhanced yield is associated with the constitutive expression of the Zea mays zmm28 gene, encoding a MADS‑box transcription factor involved in the regulation of genes associated with photosynthesis, nitrogen assimilation and carbon metabolism. Tolerance to glufosinate is conferred by expression of the pat gene from Streptomyces viridochromogenes.
Maize DAS‑4Ø278‑9 was genetically modified for tolerance to 2,4‑dichlorophenoxyacetic acid (2,4‑D) and aryloxyphenoxypropionate herbicides through expression of the Sphingobium herbicidovorans aryloxyalkanoate dioxygenase‑1 (aad‑1) gene, which inactivates these herbicides by oxidative cleavage.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-LMO-SCBD-265213-4 Organisme vivant modifié DAS-Ø1131-3 - Insect-resistant and herbicide-tolerant maizeCorteva Agriscience, Pioneer Hi-Bred International Inc. | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda)), Résistance aux herbicides (Glyphosate)
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BCH-LMO-SCBD-265294-2 Organisme vivant modifié DP-91Ø521-2 - Insect-resistant and herbicide-tolerant maizeCorteva Agriscience, Pioneer Hi-Bred International Inc. | Mannose metabolism, Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda)), Résistance aux herbicides (Glufosinate)
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BCH-LMO-SCBD-115791-1 Organisme vivant modifié DP-2Ø2216-6 - Enhanced grain yield potential and Glufosinate-ammonium resistant maizePioneer Hi-Bred International Inc. | Changements dans la physiologie et/ou la production (Production), Increased nitrogen assimilation, Increased photosynthetic capacity, Résistance aux herbicides (Glufosinate)
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BCH-LMO-SCBD-104814-2 Organisme vivant modifié DAS-4Ø278-9 - Enlist™ MaizeDow AgroSciences GmbH | Résistance aux herbicides, Tolerance to 2,4-Dichlorophenoxyacetic acid, Tolerance to aryloxyphenoxypropionate
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BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
FR
PHP88492, PHP71012, PHP79620, PHP40099, pDAS1740
EN
- Transgénèse par agrobacterium
- Pistolet biolistique / à particules
- Croisement
- Transfert direct d’ADN
- Électroporation
- Choc thermique
- Micro-injection
- Choc osmotique
- Manipulation génétique (ex. : CRISPR-Cas, etc)
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
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BCH-GENE-SCBD-103927-2 Ubiquitin 5' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Leader
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-299352-1 Cry1Da2_1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda))
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BCH-GENE-SCBD-110612-2 Ubiquitin gene terminator | Zea mays (Maize, Corn, MAIZE)Terminateur
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BCH-GENE-SCBD-265211-1 Chimeric chloroplast transit peptide TraP8 | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA) | Brassica rapa (Canola plant)Séquence signal
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BCH-GENE-SCBD-265210-2 DGT28 5-enolpiruvilshikimate- 3-phosphate synthase | Streptomyces sviceus (STRXS)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Séquence codante de protéines | Mannose tolerance,Gènes marqueurs et gènes rapporteurs sélectables
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BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)Terminateur
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BCH-GENE-SCBD-116046-1 19-kDa zein gene terminator | Zea mays (Maize, Corn, MAIZE)Terminateur
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoteur
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminateur
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BCH-GENE-SCBD-116047-3 Ubiquitin terminator | Sorghum bicolor (Sorghum)Terminateur
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BCH-GENE-SCBD-116062-1 Gamma kafarin terminator | Sorghum bicolor (Sorghum)Terminateur
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BCH-GENE-SCBD-265284-1 MMV enhancer | Mirabilis mosaic virus (MMV)Enhancer
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BCH-GENE-SCBD-265289-1 LLDV promoter | Lamium leaf distortion virus (Lamium leaf distortion-associated virus, LLDV)Promoteur
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BCH-GENE-SCBD-265290-1 Translation initiation factor 6 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-265291-1 Hydroxyproline-rich glycoprotein 5' untranslated region | Zea mays (Maize, Corn, MAIZE)Enhancer
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BCH-GENE-SCBD-265293-2 Cry1B.34 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda))
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BCH-GENE-SCBD-265292-1 Ubiquitin 1 terminator | Oryza sativa (Rice, ORYSA)Terminateur
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BCH-GENE-SCBD-115789-1 gos2 promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
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BCH-GENE-SCBD-115790-1 MADS-box transcription factor zmm28 | Zea mays (Maize, Corn, MAIZE)Séquence codante de protéines | Changements dans la physiologie et/ou la production (Production)
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-104812-3 Aryloxyalkanoate dioxygenase gene | Sphingobium herbicidovorans (SPHHE)Séquence codante de protéines | Résistance aux herbicides,Tolerance to 2,4-Dichlorophenoxyacetic acid,Tolerance to aryloxyphenoxypropionate
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BCH-GENE-SCBD-104813-4 Per5 3' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Terminateur
DNA insert from DAS‑Ø1131‑3 (vector PHP88492)
The parental maize contains two gene cassettes. The first cassette contains a Bacillus thuringiensis chimeric cry1Da2 coding sequence conferring resistance to Lepidopteran insect pests. The second cassette contains the dgt28‑epsps coding sequence from Streptomyces sviceus, which confers tolerance to glyphosate herbicide.
-Transcription of cry1Da2 is under the control of a Zea mays ubiquitin (ubi1) promoter and terminator. A ubiquitin 5′ untranslated region and intron are present at the 5′ end of the transcript to enhance gene expression. Due to the nature of the promoter, high levels of constitutive expression are expected.
The parental maize contains two gene cassettes. The first cassette contains a Bacillus thuringiensis chimeric cry1Da2 coding sequence conferring resistance to Lepidopteran insect pests. The second cassette contains the dgt28‑epsps coding sequence from Streptomyces sviceus, which confers tolerance to glyphosate herbicide.
-Transcription of cry1Da2 is under the control of a Zea mays ubiquitin (ubi1) promoter and terminator. A ubiquitin 5′ untranslated region and intron are present at the 5′ end of the transcript to enhance gene expression. Due to the nature of the promoter, high levels of constitutive expression are expected.
-Transcription of dgt28‑epsps is also under the control of a Zea mays ubiquitin promoter and terminator, with a ubiquitin leader and intron included to enhance expression. A chimeric chloroplast transit peptide (TraP8), derived from Brassica napus and Brassica rapa, directs the translated protein to the chloroplast.
Note:
- The cry1Da2 coding sequence is a chimeric sequence consisting of cry1Da2 gene encoding an insecticidal core toxin and a derivative of the cry1Ab gene.
- The TraP8 coding sequence is a chimeric sequence consisting of both Brassica napus and Brassica rapa donor organisms.
- The Cry1Da2 protein is 603 amino acids in length, including the last 9 amino acids derived from cry1Ab, and has a molecular weight of approximately 68 kDa.
- The DGT28-EPSPS protein has a length of 481 amino acids and a molecular weight of approximately 51 kDa which includes the 65-amino acid TraP8 peptide as well as a 2-amino acid linker.
- The T-DNA contains two attB recombination sites, two engineered landing pad regions and four zinc finger nuclease target recognition sites.
- Southern-by-Sequencing analysis confirmed a single insertion of the T-DNA into the maize genome and the absence of plasmid backbone sequences. Further, no unexpected insertions, deletions, rearrangements or junctions were detected.
- Southern analysis indicated stable inheritance across generations during development.
DNA insert from DP‑91Ø521‑2 (vectors PHP71012 and PHP79620)
The parental maize contains three gene cassettes introduced through site‑specific integration.
-The pmi coding sequence is under control of a Zea mays ubiquitin (ubi) promoter and a Solanum tuberosum proteinase inhibitor II (pinII) terminator. A 5' untranslated region and intron from ubi were included to enhance expression of pmi. An additional terminator (Z. mays 19-kDa zein gene terminator) was added between the gene cassettes to prevent transcriptional interference. Due to the nature of the promoter and presence of enhancers, high levels of transcription are expected.
-The pat coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. An intron from the actin 1 gene was also included to enhance transcription levels. An additional two terminators (Sorghum bicolor ubiquitin and gamma kafarin terminators) were added to prevent transcriptional interference between the gene cassettes.
-The cry1B.34 coding sequence is under control of a Lamium leaf distortion virus promoter and O. sativa ubiquitin 1 terminator. Two Mirabilis mosaic virus enhancers and Z. mays hydroxyproline-rich glycoprotein 5' untranslated region were added adjacent to the promoter to enhance expression from this gene cassette. Due to the presence of enhancers and the constitutive nature of the promoter, high-levels of transcription are expected.
-The pat coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. An intron from the actin 1 gene was also included to enhance transcription levels. An additional two terminators (Sorghum bicolor ubiquitin and gamma kafarin terminators) were added to prevent transcriptional interference between the gene cassettes.
-The cry1B.34 coding sequence is under control of a Lamium leaf distortion virus promoter and O. sativa ubiquitin 1 terminator. Two Mirabilis mosaic virus enhancers and Z. mays hydroxyproline-rich glycoprotein 5' untranslated region were added adjacent to the promoter to enhance expression from this gene cassette. Due to the presence of enhancers and the constitutive nature of the promoter, high-levels of transcription are expected.
Note:
- The size of the Z. mays ubiquitin promoter, 5' untranslated region and intron were not available at the time of record creation.
- The pat coding sequence was optimized for expression in maize.
- Cry1B.34 protein is 1,149 amino acids in length and has a molecular weight of approximately 129 kDa.
- The maize was developed using site-specific integration. The maize was initially transformed with the following plasmids to generate the site-specific "landing pad": PHP71012, PHP70594, PHP21139 and PHP21875. CRISPR-Cas expressed from PHP70594 directs the specific insertion (via homology-directed repair) of the "landing pad" into the maize genome. Following regeneration of the maize plants, a second transformation occurred where microparticle co-bombardment was used to introduce PHP79620, PHP5096, PHP21875 and PHP73572. Transient expression of FLP recombinase replaces some "landing pad" sequences with a recombination fragment. Thus, the final modified maize contains genetic elements from PHP71012 and PHP79620. All plasmids used in the transformations are briefly described below:
- PHP71012 ("Landing pad"): Z. mays genomic recognition site; Cre recombination site (loxP); Z. mays ubiquitin 1 promoter; flippase recombination site (FRT1); neomycin phosphotransferase II; pinII terminator; flippase recombination site FRT87; genomic recognition site.
- PHP70594 (transient; not integrated into genome): cas9; guide RNA
- PHP21139 (transient; not integrated into genome): Z. mays wuschel2 (wus2)
- PHP21875 (transient; not integrated into genome): Z. mays ovule developmental protein 2 (odp2)
- PHP79620 (trait genes): FRT1; pmi; pat; loxP; cry1B.34; FRT87
- PHP5096 (transient; not integrated into genome): flippase recombinase
- PHP73572 (transient; not integrated into genome): Z. mays wus2
- PHP21875 (transient; not integrated into genome): Z. mays odp2
- Southern-by-Sequencing analysis indicated that the modified maize contains a single insert. No unexpected rearrangements, junctions, additions or plasmid backbone sequences were detected.
- Southern blot analysis indicated that the insertion was stable across five generations.
DNA insert from DP202216 (vector PHP40099)
The parental maize contains two gene cassettes: Zea mays MADS-box transcription factor zmm28 and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
-Transcription of zmm28 is under control of the Z. mays gos2 promoter and the Solanum tuberosum proteinase inhibitor II gene (pinII) terminator. In addition to the zmm28 coding sequence, a Z. mays ubiquitin 1 intron is present at the 5' end of the transcript to enhance gene expression. The gos2 promoter is expected to promote constitutive expression in all plant tissues.
-Transcription of pat is under control of the Z. mays ubiquitin promoter and the pinII terminator. In addition to the pat coding sequence, the Z. mays ubiquitin 1 5' untranslated region and intron were added to the 5' end to enhance gene expression. The ubiquitin promoter is expected to promote high levels of constitutive expression in all plant tissues.
Note:
- The sequence of zmm28 is comprised of the 5’ UTR at bp 2,749-2,808 (60 bp long), coding sequence at bp 2,809-3,564 (756 bp long) and the 3’ UTR at bp 3,565-3,605 (41 bp long).
- The pat coding sequence was optimized for expression in maize.
- Southern-by-Sequencing analysis indicted that the DP202216 line contained a single T-DNA insertion containing the two gene cassettes without any rearrangements or integration of the vector backbone.
DNA inserts from DAS‑4Ø278‑9 (vector pDAS1740)
The parental maize was generated using the Whiskers mediated transformation method. It contains a single gene cassette encoding the aryloxyalkanoate dioxygenase‑1 (aad‑1) protein from Sphingobium herbicidovorans, conferring tolerance to 2,4‑dichlorophenoxyacetic acid and aryloxyphenoxypropionate herbicides. Transcription of aad‑1 is regulated by a Zea mays ubiquitin promoter and a Zea mays root‑preferential cationic peroxidase terminator. The aad-1 coding sequence was modified for plant-optimized expression.
Note:
-Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus. Additionally tests showed that there was no integration of segments from the vector backbone.
FR
- Nourriture pour les animaux
- Nourriture
- DP-91Ø521-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-Ø1131-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-4Ø278-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DP-2Ø2216-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
La presencia de cada uno de los eventos individuales puede ser determinada molecularmente mediante PCR utilizando secuencias de oligonucleótidos específicos para cada evento. En este caso, el método se basa en la detección de la presencia simultánea de cada uno de los eventos individuales a partir de ADN extraído de una única muestra biológica.
FR
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